Because the functional monomers bind to the template in a specific position within the polymer, removal of the molecule in will result in an imprint with preservation of both spatial and chemical features ( Figure 1). The cross-linking agent binds to the functional monomers upon polymerization, which embeds the template molecule in a polymer matrix. The template molecule is mixed with the functional monomer so that chemical bonds between the two are formed. In general, MIPs are made with three main reaction components: cross-linking agent, functional monomer, and template molecule. Molecularly imprinted polymers (MIPs) are used for molecular recognition because preparation is usually straight forward, they are robust, and they are chemically, mechanically, and thermally stable. These initial studies suggest there is potential for using peptide-imprinting to create specific protein-binding sites on porous inorganic surfaces, although further development of the materials is needed. However, while whole lysozyme-imprinted scaffolds showed about 4:1 preferential binding of lysozyme to RNase, peptide-imprinted scaffolds failed to show statistical significance, even though a slight preferential binding trend was present. When comparing protein- to peptide-imprinted scaffolds, similar amounts of lysozyme and RNase were bound from single protein solutions. After removing template, scaffolds were exposed to lysozyme and/or RNase A, which was used as a competitor molecule of comparable size. Whole lysozyme or the 16 residue lysozyme C peptide was imprinted into porous silica scaffolds using sol-gel processing. One method, termed the ‘epitope approach’, involves imprinting a short peptide fragment of the protein into the polymer to promote specific adsorption of the entire protein, similar to the way an antigen binds to an antibody via the epitope. We have a very pro-active approach to access challenges and will provide the safest and most efficient solutions to our clients.Of the many types of biomolecules used for molecular imprinting applications, proteins are some of the most useful, yet challenging, templates to work with. With over 15 years of project delivery in Scaffolding Solutions, we pride ourselves on having the best technical knowledge in the industry. Through the successful completion of many projects in the petrochemical and infrastructure industries, Scaffolding Solutions has always maintained an excellent relationship with our clients.
Scaffolding Solutions has worked within all types of challenging environments safely and efficiently, from confined petrochemical tanks through to under bridges and wharfs. Our first priority is to continuously improve the safety of our project delivery and provide our clients with the best products and services. Safety is the number one focus of Scaffolding Solutions. We are a preferred contractor for their current projects and look forward to working with them into the future. Scaffolding Solutions has had an ongoing working relationship with Brockman Engineering for over 15 years. We supply access solutions for maintenance and plant upgrades. Ongoing maintenance work at Tullamarine Airport, the Mobil Somerton Tank Farm and Viva Energy Bitumen Plant in Laverton North. Maintenance work and upgrading product pipelines in Newport. The tank required jacking-up to replace the floor and to add a new first strike. This 15 month project required extensive scaffolding while refurbishing SCAFFOLDING SOLUTIONS Shell Tank Farm in Cairns
0 kommentar(er)
